FIREScript® KIT
Fast and robust reverse transcriptase for wide range of applications Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures high specificity and yield wide reaction temperature from 37°C to 60°C fast 15 min reaction time a simple 2-vial kit with enzyme and reaction buffer only reaction set-up and shipment at without ice
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR). Applications